A statement from The Three Horseshoes pub said: "The Three Horseshoes will be CLOSED today Saturday 15th February following a tragic incident outside the pub last night, sorry for any inconvenience.
Pictures from the scene on Friday night showed forensic teams in white overalls examining an area of the car park, next to the pub, and carrying plastic evidence bags.
A Kent Police statement said: "Officers attended the scene along with South East Coast Ambulance Service where a woman in her 40s had suffered serious injuries.".
A white forensic screen was placed inside the parking area while a blue tarpaulin was put up next to a dark-coloured car, parked at the entrance to the car park.
Police have also established cordons around the pub in the centre of the village of Knockholt, around five miles (8km) northwest of Sevenoaks.
