During Prime Minister's Questions, Tory leader Kemi Badenoch asked whether the increase in defence spending would be used to fund the Chagos deal.
Asked about Sir Keir's response to questions about whether any of the new money will be spent on the deal, the prime minister's official spokesman said: "The uplift announced yesterday will be going on our military capabilities, technology, adopting cutting-edge capabilities that are vital to retain a decisive edge as threats rapidly evolve".
"This morning the defence secretary could not say if the Chagos deal would come out of the defence budget," she said.
Despite the move being welcomed by the main opposition parties, there have been questions as to whether the uptick in spending could be used to fund the Chagos deal, which would transfer the Indian Ocean archipelago to Mauritius after a decades-long dispute.
Sir Keir Starmer has been accused of failing to guarantee the recently announced uplift in defence spending will not be spent on the contentious Chagos Islands deal.