She is believed to be wearing a black work uniform and the trousers may be ripped and covered in mud.
She may also be wearing a brown/grey leather jacket and carrying a black handbag.
Charlotte, 35, has not been seen since leaving the Pilot Inn pub in Hardwicke, Gloucester, at around 5.25pm today.
COPS have launched an urgent hunt for a missing woman who disappeared in her work uniform.
Charlotte is described as being white, 5ft 7in in height, of a small build and with long brown hair.